Anaerobic Toxicity

Methanogenic Inhibitors

Compound
 Category

Anaerobic
 Toxicity

Comments


Polysaccharides &
 Simple sugars

   

Proteins &
 Aminoacids

   

Fats &
 Long chain fatty acids (LCFA)

+

IC50 for LCFA = 500-1.250 mg/l

Simple alcohols

  
Volatile fatty acids (VFA)

+


Non-toxic at pH 7.0-8.0
 Toxicity is pH-dependent
 Non-ionized form is toxic (free VFA)
 Acetate and propionate:
 (IC50 = 6-16 mg free VFA/l)
Cyanide (CN-)

+++


IC50=  1 mg/l (unadapted sludge)
 IC50= 25 mg/l (adapted sludge)
Formaldehyde

+++


IC50= 100 mg/l (unadapted sludge)
 IC50= 400 mg/l (adapted sludge)

Inorganic S compounds
  • Sulfide

++

Toxicity is pH-dependent
 Non-ionized form is toxic (free H2S)
 IC50 = 250 mg free S-H2S//l
  • Sulfate
Only at the very high concentrations
 IC50 = 3.300 mg S-SO4-2/l
  • Sulfite
+++IC50 = 50 mg S-SO3-2/l
Ammonia

++


Toxicity is pH-dependent
 Non-ionized form is toxic (free NH3)
 IC50 = 50 mg free N-NH3/l
Heavy metals

+++/++


Some heavy metals are very toxic
 Soluble form is toxic. Some metal are very toxic (eg. IC 50= 10-75 mg soluble Cu+2/l)

In anaerobic systems, heavy metals are often detoxified by precipitation with carbonates and sulfides.

Salts
  (eg. Na+, K+, Ca2+)

+


Only at high concentrations
 IC50 = 4.700-7.600 mg/l

Aromatic compounds
  • Aromatic hydrocarbons

+

eg. IC50 benzene =        1970 mg/l
      IC50 ethylbenzene =  340 mg/l
  • Phenol

+

IC50= 1300 mg/l
  • Benzoic acid
IC50>> 4.250 mg/l
  • Benzaldehyde

+

  
  • Chlorinated phenols
+++IC50 pentachlorophenol = 1 mg/l
  • Chlorobenzenes

+++

  
  • Nitro-aromatic compounds

+

eg. IC50 nitrobenzene = 10 mg/l
  • Aromatic amines

++

eg. IC50 aniline = 900 mg/l

Aliphatic compounds
  • Chlorinated aliphatic hydrocarbons

+++

eg. chloroform
 IC50= 0.5 mg/l (unadapted sludge)
 IC50= 45 mg/l (adapted sludge)

Surfactants
  • Cationic and anionic detergents

++

  IC50 = 20-50 mg/l

Azo dyes

─/+

 Highly variable

Phytochemicals
  • Lignin

High molecular lignin is non-toxic
  • Lignin monomeric compounds

+/++

eg. IC50 guaiacol =   2.200 mg/l
      IC50 isoeugenol =   264 mg/l
  • Volatile terpenes
+++eg. IC50 pinene = 180 mg/l
  • Wood resin
+++eg. IC50 abietane = 140 mg/l
  • Tannins
++eg. IC50 bark tannins = 350 mg/l
 High molecular weight tannins are non-toxic

To make readers understand well, author should give simple description of IC50, and symbols of “+++, ++, +, and –“.

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